Mouselines
The Mouselines pages are available for all of your colony
data management. Some common workflow processes now have their own
automated pages, simplifying your day and freeing up your time.
Features You Know and Love
Click on image for a more detailed view.
Alleles
We want our genetic information to be as accurate as
possible. All of the allele information is in its own section,
including important information that didn't previously have a
clear place, in LIMS alleles can be shared between different
passports. This means that complex genetic information only
needs to be entered once for each allele you're working with,
greatly simplifying the process of performing multiple crosses
and making it much easier for you to make sure that all the
genetic information is accurate and complete.
Where to Find this Feature: Mouseline > Allele Search ( > View Allele ) and View Mouseline > View Allele
Click on image for a more detailed view.
Genotyping Assays
The genotyping assays section on an allele has been
improved to make it easier for different labs to add and update.
As long as you can view the allele, you may add assays to it.
These assays will be identified by the name and lab, will be
visible to others who can see the alleles, but will only be
editable by members of the lab. Primer sets are also no longer
limited to two sets.
Where to Find this Feature: Mouseline > Allele Search ( > View Allele ) and View Mouseline > View Allele > Genotyping
Click on image for a more detailed view.
Zygosity Tracking
Zygosity (heterozygous, homozygous, etc) is tracked on a
per-mouse basis. This information will stay with a mouse forever
once it has been entered, so you no longer need to worry about
copying that information from cage to cage or losing the
information once the mouse has been sacrificed. You can also
specify zygosity for as many alleles as the mouse has, although
the number printed on the physical cage card will still be
limited for space reasons. Alleles can be assigned "shortform
names" which will be used in place of the full name.
Where to Find this Feature: View Cage and View Mouse
Click on image for a more detailed view.
Per-lab Mouseline Common Names
Since some labs refer to a mouseline as one name, while
another lab might refer to the same mouseline by a totally
different name, it can be confusing to try to share information.
A mouseline can now have a common name associated with it for
each AUP that it is on. This name will show on cages and mice
associated with that AUP. Labs can use this common name as an
easier alternative to the more technical standard nomenclature
which should also be included on the passport.
Where to Find this Feature: View AUP > Mouselines
Click on image for a more detailed view.
Welfare
Since one of our primary concerns at TCP is the health and
welfare of our animals, we've expanded and standardized our
approach to mouseline welfare information. We have a set of
standard welfare terms and categories that can be listed under
any mouseline passport. By standardizing the terms, we can help
encourage their use by making them easier to enter into the
passport, and we can also remove some confusion that can happen
when unfamiliar terms are used. For some specific terms, an icon
will even be printed on each cage card associated with that
mouseline to ensure that staff working with those mice are aware
of any potential issues. Welfares will need to be entered if a
welfare assessment was done and welfare issues were found.
Where to Find this Feature: View Mouseline > Welfare
Click on image for a more detailed view.
Attachments
You can attach files to a mouseline passport and alleles.
These files can be viewed by anyone who can view the passport.
For instance, you could attach gene sequencing information,
pictures of welfare-related issues, or published research. If
the attachments associated with the Mouseline are searchable
PDFs, the contents of the documents are included in the
Mouseline search if the Document Contents option is selected in
the Column Options dropdown.
Where to Find this Feature: View Mouseline > Attachments and View Allele > Attachments
Click on image for a more detailed view.
Combine Mouselines
Most new mouseline passports are the result of crosses
between mice already in TCP. With the new allele system, you can
now perform this common task with a minimum of effort by using
the combine mouselines feature. While viewing one mouseline,
click the Combine button to select a second mouseline to combine
the current one with. Pressing Next will then take you to a page
that will let you create a new passport containing all of the
alleles from both of the parent passports. If you need to add or
remove some of the alleles, you can do that as well. Much faster
and more accurate than cloning an existing mouseline, the
combine feature saves you valuable time.
Where to Find this Feature: View Mouseline > Combine (in the Action Panel)
Click on image for a more detailed view.
MGI Integration
To help reduce data entry and improve accuracy, we have
arranged with the Mouse Genome Informatics group at The Jackson
Laboratory to access the MGI allele database in realtime from
within LIMS. This means that, while filling out genetic
information as part of a passport, you can now search for the
affected gene and allele in the MGI database without leaving
LIMS. We can then show some of the basic information associated
with that gene and allele, as well as the direct link to the MGI
page showing detailed information.
Where to Find this Feature: View Allele > MGI
Click on image for a more detailed view.
Find Similar Mouselines
Since we are a shared facility, it's sometimes the case
that multiple passports are entered for the same mouseline.
We've added a section on the mouseline page that shows other
passports that are similar to the current one based on which
alleles they contain. When you're reviewing your passports, you
can check to make sure that there aren't any duplicates.
Duplicate passports reduce our ability to collaborate and tend
to cause one or both passports to not have a complete picture of
all the information we have on that mouseline.
Where to Find this Feature: View Mouseline > Similar Mouselines (in Related Entities Panel)
Click on image for a more detailed view.
Active Cage Listing
When viewing a mouseline, the number of active cages for
that mouseline is displayed for the selected filter. TCP
Managers can access this information for all AUPs, allowing
staff to quickly check if a mouseline is currently active in the
facility or not. This can help prevent unnecessary
rederivations.
Where to Find this Feature: View Mouseline
Click on image for a more detailed view.
Mouseline Lifecycle
Mouselines have a status to indicate their approval status
as well as 4 flags.
Statuses -
- Under Review - Changes to the Mouseline are being
reviewed by TCP.
- Approved - Mouseline has been reviewed and approved
for use at TCP.
- Modified - Mouseline has been modified since it was
initially approved.
- Archived - Mouseline is no longer in use at TCP.
Mouselines that are ARCHIVED are not included in Mouseline
dropdowns.
Flags -
- Cryopreserved - Mouseline has been cryopreserved. The
Mouseline may or may not be archived. The Mouseline should be
Complete.
- Complete - Mouseline includes all the required
information. This field is modified by Mouseline
Administrators.
- Confidential - Mouselines that are CONFIDENTIAL are
only visible to individuals that are associated with AUPs that
are associated with the Mouseline. Mouseline confidentiality
is no longer determined by the confidentiality of the
associated alleles, but by its own confidentiality flag.
- Delayed Weaning - Flag is set if the Mouseline
requires delayed weaning of pups.
Currently, not all APPROVED or MODIFIED Mouselines are
Complete but we are working to fix this. They must be Complete
before they are Crypreserved.
Where to Find this Feature: View Mouseline > Admin
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